A follow-up study of children born after intracytoplasmic sperm injection (ICSI) with epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI

摘要: The aim of this prospective follow-up study children born after intracytoplasmic sperm injection (ICSI) was to compile data on karyotypes, congenital malformations, growth parameters and developmental milestones in order evaluate the safety new technique. design included karyotyping parents their agreement genetic counselling prenatal diagnosis it based a physical examination child at Centre for Medical Genetics ages 2 months, 1 year years, where major minor malformations psychomotor evolution are recorded. Here we describe first 57 from 40 ICSI pregnancies with epididymal spermatozoa (group 1), 50 34 testicular 2) 58 48 replacement cryopreserved embryos 3). Parental karyotypes were obtained only 72/246 (29%) all normal. Prenatal determined total 70 samples (40%): 21 group 1, 15 3. In last abnormal 47,XXY (5.8%) no structural aberrations found. This increase de-novo sex-chromosomal has already been described regard 877 carried out our is probably linked directly characteristics infertile men treated rather than procedure itself. Major defined as those causing functional impairment or requiring surgical correction, observed four children: two spermatozoa, one cryopreservation. No particular malformation disproportionally frequent. examinations months (107/ 161 66.5%) (37/161 22.9%), additional anomalies observed. Lost rate 33.5%. These observations limited number do not suggest higher incidence diseases imprinting, nor they malformations. still should be further completed by others collaborative efforts. meanwhile patients told about available before any treatment: there appears some risk transmitted chromosomal aberrations, de-novo, mainly transmitting fertility problems offspring. Patients also reassured that until now seems embryos.