Purification of hepatocyte couplets by centrifugal elutriation

作者: Joanne C. Wilton , Delyth E. Williams , Alastair J. Strain , Rosemary A. Parslow , J. Kevin Chipman

DOI: 10.1002/HEP.1840140129

关键词:

摘要: An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the loaded onto elutriator, 87% were eluted at medium flow rates 60 to 80 ml/min a rotor speed 1,100 rpm; cells in this range maintained viability more than 95%. Peak fractions 84.5% +/- 2.5%. After elutriation, retained ability secrete fluorescent cholephiles into sealed canalicular vacuoles. The can now be used hepatobiliary and hepatotoxicity studies not possible with preparations which they are minor components.

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