[23] Cell separation by elutriation: Major and minor cell types from complex tissues

摘要: Publisher Summary This chapter describes the cell separation by elutriation. The definition of elutriation is or removal washing. Centrifugal cells accomplished in a rotor that allows counterflow medium through chamber opposing centrifugal force; balance and force underlies separation. design set rotating seals perfusion spinning critical step development elutriator rotor. advantages are large numbers can be rapidly processed. technique reproducible use any medium, rather than expensive possibly toxic media necessary for density gradient separations. Cell viability, general, maintained, although there some sensitive to shear forces encountered. Another major advantage bacteria, with their small size, flushed out during initial wash; therefore, when sterile used properly cleansed, fractions result which suitable culture.