Mycobacterium bovis with different genotypes and from different hosts induce dissimilar immunopathological lesions in a mouse model of tuberculosis.

作者: D. Aguilar León , M. J. Zumárraga , R. Jiménez Oropeza , A. K. Gioffré , A. Bernardelli

DOI: 10.1111/J.1365-2249.2009.03923.X

关键词:

摘要: With the hypothesis that genetic variability of Mycobacterium bovis could influence virulence and immunopathology, five M. strains were selected from an epidemiological study in Argentina on basis their prevalence cattle occurrence other species. We then determined immunopathology evoked by these a well-characterized mouse model progressive pulmonary tuberculosis. The reference strain AN5 was used as control. BALB/c mice infected with this showed 50% survival after 4 months infection, moderate bacillary counts lung. Two weeks inoculation, it induced strong inflammatory response numerous granulomas pneumonia. In contrast, 04-303, isolated wild boar, most lethal its striking feature sudden pneumonia extensive necrosis. Strain 04-302, also boar but different spoligotype, similar pathology to lesser extent. 534, V2 (both cattle) 02-2B (from human) less virulent, permitting higher infection limited tissue damage. human isolates rapid, high stable expression interferon (IFN)-gamma inducible nitric oxide synthase (iNOS). more virulent lower IFN-gamma, tumour necrosis factor-alpha iNOS. Interestingly, very low murine beta defensin (mBD-4); whereas, control anti-microbial peptide, peaking at day 120. mBD-4 during early infection. Thus, reported clinical tuberculosis, variable virulence. This can be attributed induction pattern immune response.

参考文章(42)
R Hernandez-Pando, H Orozcoe, A Sampieri, L Pavon, C Velasquillo, J Larriva-Sahd, JM Alcocer, MV Madrid, Correlation between the kinetics of Th1, Th2 cells and pathology in a murine model of experimental pulmonary tuberculosis. Immunology. ,vol. 89, pp. 26- 33 ,(1996)
M. J. Zumárraga, A. Bernardell, R. Bastida, V. Quse, J. Loureiro, A. Cataldi, F. Bigi, A. Alito, M. Castro Ramos, S. Samper, I. Otal, C. Martin, M. I. Romano, Molecular characterization of mycobacteria isolated from seals. Microbiology. ,vol. 145, pp. 2519- 2526 ,(1999) , 10.1099/00221287-145-9-2519
Alicia Aranaz, Ernesto Liébana, Enrique Gómez-Mampaso, Juan C. Galán, Debby Cousins, Arturo Ortega, Jesús Blázquez, Fernando Baquero, Ana Mateos, Guillermo Súarez, Lucas Domínguez, Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain. International Journal of Systematic and Evolutionary Microbiology. ,vol. 49, pp. 1263- 1273 ,(1999) , 10.1099/00207713-49-3-1263
Rogelio Hernandez-Pando, Diana Aguilar, Maria Luz Garcia Hernandez, Hector Orozco, Graham Rook, Pulmonary tuberculosis in BALB/c mice with non‐functional IL‐4 genes: changes in the inflammatory effects of TNF‐α and in the regulation of fibrosis European Journal of Immunology. ,vol. 34, pp. 174- 183 ,(2004) , 10.1002/EJI.200324253
R. Hernández-Pando, T. Schön, E.H. Orozco, J. Serafin, I. Estrada-García, Expression of inducible nitric oxide synthase and nitrotyrosineduring the evolution of experimental pulmonary tuberculosis Experimental and Toxicologic Pathology. ,vol. 53, pp. 257- 265 ,(2001) , 10.1078/0940-2993-00182
Wladimir Malaga, Patricia Constant, Daniel Euphrasie, Angel Cataldi, Mamadou Daffé, Jean-Marc Reyrat, Christophe Guilhot, Deciphering the Genetic Bases of the Structural Diversity of Phenolic Glycolipids in Strains of theMycobacterium tuberculosisComplex Journal of Biological Chemistry. ,vol. 283, pp. 15177- 15184 ,(2008) , 10.1074/JBC.M710275200
J.M. Pollock, J.D. Rodgers, M.D. Welsh, J. McNair, Pathogenesis of bovine tuberculosis: The role of experimental models of infection Veterinary Microbiology. ,vol. 112, pp. 141- 150 ,(2006) , 10.1016/J.VETMIC.2005.11.032