Towards a semi-perennial culture of Stevia rebaudiana (Bertoni) Bertoni under temperate climate: effects of genotype, environment and plant age on steviol glycoside content and composition

摘要: Stevia rebaudiana (Bertoni) Bertoni is a perennial shrub native to Paraguay whose leaves accumulate sweetening compounds of high value: steviol glycosides (SVglys). These diterpenoids differ in the number (1–4) and nature sugar units bound skeleton: mainly glucose but also rhamnose xylose. The two main SVglys are stevioside (ST; 3 units) rebaudioside A (RA, 4 units). Along with an evaluation genotypic variability for content composition, this study investigates winter survival effect cultivation on S. genotypes through 2-year experiment conducted locations. first year, population 96 was examined nursery field (site A) subset five grown trial B). surviving (16 site 5 B) were studied following alongside new plantings same lines (8 showed SVgly (from 45.7 186.2 mg g−1 DM) composition along four distinct clusters. There no correlation between total composition. Genotypes without RA had other more than three conjugated sugars. varied among environments generally increased 1- 2-year-old plants, while remained stable. This that breeding temperate conditions would allow increase productivity semi-perennial stevia production.