Induction of the reelin promoter by retinoic acid is mediated by Sp1.
作者： Ying Chen , Marija Kundakovic , Roberto C. Agis-Balboa , Graziano Pinna , Dennis R. Grayson
DOI: 10.1111/J.1471-4159.2007.04797.X
关键词:
摘要: We have previously described the cloning of human reelin promoter and provided evidence that it is regulated, in part, through changes methylation. Results from our current studies provide a more detailed analysis this interactions transcription factors Sp1 paired box gene 6 (Pax6) with their recognition sites. The was studied NT2 cells which are neuroprogenitor line undergoes differentiation vitro. examined mRNA induction following 6-day treatment these retinoic acid (RA). Deletion site-directed mutations showed functionally relevant sequences necessary for regulation. Gel-shift assays demonstrated main site action lies within closely packed ( approximately 25 bp) region likely bind, possibly forming DNA/protein complex. Based on results, appears RA-induces expression critical resides adjacent to Pax6 multisite enhancer region. show RA accompanied by higher amounts binding Finally, we while prevent RA-mediated induction, similar do not. data suggest plays role modulating expression, not absolutely required RA.
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