S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component.

摘要: We have isolated and functionally characterized a multicomponent Oct-1 coactivator, OCA-S which is essential for S phase-dependent histone H2B transcription. The p38 component of binds directly to Oct-1, exhibits potent transactivation potential, selectively recruited the promoter in phase, phase-specific transcription vivo vitro. Surprisingly, represents nuclear form glyceraldehyde-3-phosphate dehydrogenase, binding as well function, stimulated by NAD(+) but inhibited NADH. also interacts with NPAT, cyclin E/cdk2 substrate that broadly involved gene These studies thus link transcriptional machinery cell cycle regulators, possibly cellular metabolic state (redox status), set stage underlying mechanisms basis coordinated expression coupling DNA replication.