Early Secreted Antigenic Target of 6-kDa of Mycobacterium tuberculosis Stimulates IL-6 Production by Macrophages through Activation of STAT3.
作者： Bock-Gie Jung , Xisheng Wang , Na Yi , Justin Ma , Joanne Turner
DOI: 10.1038/SREP40984
关键词:
摘要: As early secreted antigenic target of 6 kDa (ESAT-6) Mycobacterium tuberculosis (Mtb) is an essential virulence factor and macrophages are critical for infection immunity, we studied ESAT-6 stimulated IL-6 production by macrophages. significantly higher secretion murine bone marrow derived (BMDM) compared to culture filtrate protein 10 kDa (CFP10) antigen 85A. Polymyxin B, LPS blocker, did not affect macrophage production. but Pam3CSK4 induced TLR2 knockout BMDM. phosphorylation DNA binding STAT3 this was blocked inhibitors rapamycin. suppressed ESAT-6-induced transcription without affecting cell viability. This confirmed silencing in Blocking neither IL-6Rα/IL-6 nor IL-10 affected activation Infection BMDM human with Mtb esat-6 deletion diminished reduced wild type complemented strains. Administration CFP10 expression the mouse lungs, consistent ESAT-6, phosphorylated-STAT3 Mtb-infected lungs. We conclude that stimulates through activation.
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