Immobilization of acetylcholinesterase on functionalized SBA-15 mesoporous molecular sieve for detection of organophosphorus and carbamate pesticide
作者： Yin Rui , Xiaomei Wu , Baodi Ma , Yi Xu
DOI: 10.1016/J.CCLET.2017.10.033
关键词:
摘要: Abstract The immobilization of acetylcholinesterase (AChE) on amino functionalized SBA-15 mesoporous sieves (NH 2 -SBA-15) was explored for detection organophosphorus and carbamate pesticides. Several methods have been studied the “adsorption-crosslinking” is found to be best one. Firstly, AChE bovine serum albumin (BSA) were adsorbed in turn surface NH -SBA-15 by electrostatic adsorption, then glutaraldehyde added cross-linking group AChE. showed around 95% efficiency specific activity immobilized (AChE-NH reached 130% relative free under optimal conditions. It AChE-NH retained 94.0% 82.8% its initial after 60 days at 4 °C 25 °C, respectively. remained 55.0% 6 times recycling. During pesticide, wider linear range much lower limit compared with current method good recovery (93.8%-109.3%) low RSD ( could exploited as a fast, sensitive low-cost biocatalyst towards pesticides residues which stored room temperature long time.
sci-hub.se 参考文章(43)
Xiu-Yu Sun, Peng-Zhang Li, Bing Ai, Yue-Bo Wang, Surface modification of MCM-41 and its application in DNA adsorption Chinese Chemical Letters. ,vol. 27, pp. 139- 144 ,(2016) , 10.1016/J.CCLET.2015.08.008
Ghasem Rezanejade Bardajee, Marzieh Mohammadi, Hasan Yari, Aseyeh Ghaedi, None, Simple and efficient protocol for the synthesis of benzoxazole, benzoimidazole and benzothiazole heterocycles using Fe(III)–Schiff base/SBA-15 as a nanocatalyst Chinese Chemical Letters. ,vol. 27, pp. 265- 270 ,(2016) , 10.1016/J.CCLET.2015.10.011
Rocio B. Dominguez, Gustavo A. Alonso, Roberto Muñoz, Akhtar Hayat, Jean-Louis Marty, Design of a novel magnetic particles based electrochemical biosensor for organophosphate insecticide detection in flow injection analysis Sensors and Actuators B-chemical. ,vol. 208, pp. 491- 496 ,(2015) , 10.1016/J.SNB.2014.11.069
Hayrettin Tümtürk, Ferat Şahin, Gökhan Demirel, A new method for immobilization of acetylcholinesterase Bioprocess and Biosystems Engineering. ,vol. 30, pp. 141- 145 ,(2007) , 10.1007/S00449-006-0106-8
Jie Fan, Chengzhong Yu, Feng Gao, Jie Lei, Bozhi Tian, Limin Wang, Qian Luo, Bo Tu, Wuzong Zhou, Dongyuan Zhao, Cubic Mesoporous Silica with Large Controllable Entrance Sizes and Advanced Adsorption Properties Angewandte Chemie International Edition. ,vol. 42, pp. 3146- 3150 ,(2003) , 10.1002/ANIE.200351027
Yaodong Zhang, Yanan Cai, Zongli Qi, Lu Lu, Yunxia Qian, DNA-templated silver nanoclusters for fluorescence turn-on assay of acetylcholinesterase activity. Analytical Chemistry. ,vol. 85, pp. 8455- 8461 ,(2013) , 10.1021/AC401966D
Chenghong Lei, Yongsoon Shin, Jun Liu, Eric J. Ackerman, Entrapping enzyme in a functionalized nanoporous support. Journal of the American Chemical Society. ,vol. 124, pp. 11242- 11243 ,(2002) , 10.1021/JA026855O
Kayambu Kannan, Raksh Vir Jasra, Immobilization of alkaline serine endopeptidase from Bacillus licheniformis on SBA-15 and MCF by surface covalent binding Journal of Molecular Catalysis B-enzymatic. ,vol. 56, pp. 34- 40 ,(2009) , 10.1016/J.MOLCATB.2008.04.007
Irina Matlahov, Yasmin Geiger, Gil Goobes, Trapping RNase A on MCM41 pores: effects on structure stability, product inhibition and overall enzymatic activity Physical Chemistry Chemical Physics. ,vol. 16, pp. 9031- 9038 ,(2014) , 10.1039/C3CP55520H
A. Crew, D. Lonsdale, N. Byrd, R. Pittson, J.P. Hart, A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides Biosensors and Bioelectronics. ,vol. 26, pp. 2847- 2851 ,(2011) , 10.1016/J.BIOS.2010.11.018