Identification and localization of allergenic determinants on grass group I antigens using monoclonal antibodies.

摘要: Epitopes recognized by five mAb which block the binding of human IgE antibodies to grass group I (GpI) Ag were characterized and partially mapped. Site specificity studies defined four apparently non-overlapping blocking antibody sites on meadow fescue GpI molecule, Fes e I. One these (site A) was localized a 14,000 m.w. fragment designated P3 generated CNBr cleavage purified The peptide possessed as well other epitopes (non-site 19 anti-GpI mAb. All reactive cross-reactive determinants found isolated from different grasses suggesting that is conserved portion molecules. used immunize mice induced reacted with intact all 5 currently being studied in this laboratory.