LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium

摘要: Summary We report the first investigation of binding Salmonella enterica LeuO LysR-type transcription regulator to its genomic targets in vivo. Chromatin-immunoprecipitation-on-chip identified 178 sites on chromosome S. enterica serovar Typhimurium strain SL1344. These were distributed across both core and horizontally acquired genome, included housekeeping genes known contribute virulence. Sixty-eight co-bound by global repressor protein, H-NS. Thus, while may function as an H-NS antagonist, these functions are unlikely involve displacement RNA polymerase bound 173 targets, consistent with being a regulator. two classes genes, those that not site analysis revealed logo conforming TN11A motif common factors. It differed some details from we composed for Escherichia coli sites; 1263 1094 locations predicted S. Typhimurium SL1344 E. coli MG1655 genomes respectively. Despite differences composition, many target species. is likely be more important than previously suspected.