Controlled encapsidation of gold nanoparticles by a viral protein shell.

摘要: Icosahedral virus capsids demonstrate a high degree of selectivity in packaging cognate nucleic acid components during assembly. This specificity, when integrated as part nanotechnological protocol, has the potential to encapsidate wide array foreign materials for delivery therapeutics or biosensors into target cells. Red clover necrotic mosaic (RCNMV) exclusively packages two genomic ssRNAs initiated by specific protein:RNA interaction between RCNMV coat protein (CP) and viral RNA origin assembly (OAS) element. In present work, an oligonucleotide mimic OAS sequences is attached Au nanoparticles recognition signal initiate virion-like CP. Covalent linkage functions trigger encapsidation demonstrates that cargo can be packaged virions.