Establishment and Characterization of Patient-Derived Xenografts (PDXs) of Different Histology from Malignant Pleural Mesothelioma Patients

摘要: Background: Malignant pleural mesothelioma (MPM) is a very aggressive tumor originating from mesothelial cells. Although several etiological factors were reported to contribute MPM onset, environmental exposure asbestos certainly major risk factor. The latency between (or asbestos-like fibers) and onset long. continues be with poor prognosis despite the introduction of new therapies including immunotherapy. One problems low number preclinical models able recapitulate features human tumors. This impacts possible discovery treatments combinations. Methods: In this work, we aimed generate patient-derived xenografts (PDXs) patients covering three histotypes (epithelioid, sarcomatoid, mixed) occurring in clinic. To do this, obtained fresh tumors biopsies or pleurectomies, samples subcutaneously implanted immunodeficient mice within 24 h. Results: We successfully isolated different PDXs particularly concentrated our efforts on histotypes. that grew compared well histologically origin, showed stable growth response cisplatin, as was observed Conclusions: These are helpful testing drugs combinations that, if successful, could rapidly translate clinical setting.