The effect of trace amounts of tissue factor on thrombin generation in platelet rich plasma, its inhibition by heparin

摘要: Amounts of human brain thromboplastin that do not stimulate thrombin generation in platelet poor plasma, were shown to advance by about 4 min an explosive formation occurs after recalcification the presence blood platelets. This synergistic effect is inhibited specific inhibitor hirudin and mimicked adding low concentrations (less than 5 nM) rich plasma. It our conclusion small amounts thrombin, generated under influence induced procoagulant activity most likely mainly due phospholipids. Heparin inhibits this retards formation. does not, however, diminish peak amount eventually formed, because heparin neutralizing material released from activated platelets quenches effect.