Detection and analysis of DNA methylation by pyrosequencing.
作者： Hilary A. A. Colyer , Richard N. Armstrong , Daniel J. Sharpe , Ken I. Mills
DOI: 10.1007/978-1-61779-612-8_17
关键词:
摘要: Pyrosequencing is a "sequencing by synthesis" technique which can be used to quantify DNA methylation at specific CpG sites within the target region of interest. Biotin labelled polymerase chain reaction (PCR) products form template for base-pair nucleotide incorporation causing light emitting cascade resulting in formation pyrogram and calculation percentage each site. Prior pyrosequencing, it essential bisulphite-convert sample then perform locus-specific PCR One primers needs biotinylated separate sequencing primer required pyrosequencing itself.
springer.com
springerlink.com参考文章(9)
Jörg Tost, Ivo Glynne Gut, Analysis of gene-specific DNA methylation patterns by pyrosequencing technology. Methods of Molecular Biology. ,vol. 373, pp. 89- 102 ,(2007) , 10.1385/1-59745-377-3:89
Pål Nyrén, The history of pyrosequencing. Methods of Molecular Biology. ,vol. 373, pp. 1- 14 ,(2007) , 10.1385/1-59745-377-3:1
Nicola Klare, Michael Seeger, Katharina Janek, Peter R. Jungblut, Burkhardt Dahlmann, Intermediate-type 20 S Proteasomes in HeLa Cells: “Asymmetric” Subunit Composition, Diversity and Adaptation Journal of Molecular Biology. ,vol. 373, pp. 1- 10 ,(2007) , 10.1016/J.JMB.2007.07.038
Ruethairat Sriraksa, Patimaporn Chaopatchayakul, Patcharee Jearanaikoon, Chanvit Leelayuwat, Temduang Limpaiboon, Verification of complete bisulfite modification using Calponin-specific primer sets. Clinical Biochemistry. ,vol. 43, pp. 528- 530 ,(2010) , 10.1016/J.CLINBIOCHEM.2009.11.005
M. Frommer, L. E. McDonald, D. S. Millar, C. M. Collis, F. Watt, G. W. Grigg, P. L. Molloy, C. L. Paul, A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 1827- 1831 ,(1992) , 10.1073/PNAS.89.5.1827
Susan J Clark, Aaron Statham, Clare Stirzaker, Peter L Molloy, Marianne Frommer, DNA methylation: bisulphite modification and analysis. Nature Protocols. ,vol. 1, pp. 2353- 2364 ,(2006) , 10.1038/NPROT.2006.324
Debra Ting Hsiung, Carmen J. Marsit, E. Andres Houseman, Karen Eddy, C. Sloane Furniss, Michael D. McClean, Karl T. Kelsey, Global DNA Methylation Level in Whole Blood as a Biomarker in Head and Neck Squamous Cell Carcinoma Cancer Epidemiology, Biomarkers & Prevention. ,vol. 16, pp. 108- 114 ,(2007) , 10.1158/1055-9965.EPI-06-0636
Jörg Tost, Ivo G Gut, DNA methylation analysis by pyrosequencing Nature Protocols. ,vol. 2, pp. 2265- 2275 ,(2007) , 10.1038/NPROT.2007.314
N. B. Gusev, Some properties of caldesmon and calponin and the participation of these proteins in regulation of smooth muscle contraction and cytoskeleton formation Biochemistry. ,vol. 66, pp. 1112- 1121 ,(2001) , 10.1023/A:1012480829618