Preparation and bivariate analysis of suspensions of human chromosomes.
作者： G. J. van den Engh , B. J. Trask , J. W. Gray , R. G. Langlois , L.-C. Yu
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摘要: Chromosomes were isolated from a variety of human cell types using HEPES-buffered hypotonic solution (pH 8.0) containing KCl, MgSO4, dithioerythritol, and RNase. The chromosomes by this procedure could be stained with fluorescent stains including propidium iodide, chromomycin A3, Hoechst 33258. Addition sodium citrate to the was found improve total fluorescence resolution. High-quality bivariate vs. distributions obtained for fibroblast strain, colon carcinoma line, peripheral blood lymphocyte cultures. Good flow karyotypes also primary amniotic given made at different times dye concentrations varying over fourfold ranges, show little variation in relative peak positions chromosomes. size DNA ranges 20 50 kilobases. described isolation is simple, it yields high-quality karyotypes, can used prepare clinical samples.
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