A novel flow cytometric method for the quantification of p53 gene expression.

摘要: We describe a rapid and simple flow cytometry technique for the detection quantification of p53 in several human cell lines, including an adenocarcinoma line (SW 626) having mutant (m) p53, pre-B leukemia (NALM-6) wild-type (wt) p53. By introducing second antibody coupled to RPE-fluorescence, discrimination between control specific peaks was improved over that achieved with methods used previously. To quantify content molecules cells, we series beads capability bind mouse monoclonal IgG antibodies. content, expressed as binding capacity (ABC), directly quantified from logarithmic scattergrams; results were reproducible all lines tested. Flow cytometric compared those standard immunocytochemistry method routinely found be correlation. Furthermore, data also reflect ELISA determinations. In summary, showed first time (i) can clearly detected by various (ii) quantitated terms number per cell, (iii) it easily monitored function after stimulation. Cytometry 31:180–186, 1998. © 1998 Wiley-Liss, Inc.