Starvation-induced cross protection against heat or H2O2 challenge in Escherichia coli.

摘要: Glucose- or nitrogen-starved cultures of Escherichia coli exhibited enhanced resistance to heat (57 degrees C) H2O2 (15 mM) challenge, compared with their exponentially growing counterparts. The degree increased the time for which cells were starved prior 4 h starvation providing maximal protection. Protein synthesis during was essential these cross protections, since chloramphenicol addition at onset prevented development thermal oxidative resistance. Starved also demonstrated stronger and than did adapted heat, H2O2, ethanol challenge. Two-dimensional gel electrophoresis 35S-pulse-labeled proteins showed that subsets 30 glucose synthesized adaptation; three common all stresses. Most among previously identified Pex (J.E. Schultz, G. I. Latter, A. Matin, J. Bacteriol. 170:3903-3909, 1988), are independent cyclic AMP positive control induction starvation. Induction dependent on not important in a delta cya strain E. K-12 same as wild-type parent both growth