CONTROL OF CELL DEATH (APOPTOSIS) BY DIETHYLSTILBESTROL IN AN ESTROGEN-DEPENDENT KIDNEY TUMOR

摘要: The role of cell death as a determinant for tumor growth and regression was studied using an estrogen-dependent, transplantable kidney designated H301. H301 cells were injected s.c. into diethylstilbestrol(DES)-treated male Syrian hamsters developed solid tumors 1-2 g within 2-3 weeks. Upon withdrawal estrogen the regressed by 80-90% 4 days. Mitoses, necrotic areas single-cell indicated small, condensed residues, counted in hematoxylin eosin stained histological sections tumors. Coincident with after DES withdrawal, mitotic activity decreased approximately 90%; rate increased (by 2-fold at its maximum). incidence not affected withdrawal. re-treatment resulted reduction 80% 8 h. Mitotic 24 h to level observed before Again, did change. As result, re-grew their previous size 2 days resumption treatment. These results led following conclusions: (i) treatment inhibits enhances cells. (ii) Both this functional property morphology characterize apoptosis. (iii) Estrogen-dependent determines, addition mitosis necrosis, (iv) This experimental model may provide useful tool study interaction potential anti-tumor drugs apoptosis neoplasia.