Embryo-dependent induction of uterine receptivity assessed by an in vitro model of implantation in mice.
作者： Masahide Shiotani , Yoichi Noda , Takahide Mori
DOI: 10.1095/BIOLREPROD49.4.794
关键词:
摘要: To investigate the molecular basis of initial interactions between trophoblast and uterine epithelium at implantation, we developed an in vitro model for implantation mice using primary organ cultures as substrate. In this model, embryos attached to endometrial explants within 60 min. We were able observe attachment real time. Using investigated strips cultured from pregnant mice, pseudopregnant that had received hCG injections, whose utero-tubal junctions been ligated on Day 2 pregnancy. The serum levels progesterone also compared. percentages Days 1-4 low (0.0-15.0%). However, by 5-7, increased markedly (66.8-74.8%). These significantly higher than those observed (0.0-18.0%) injections horns 5 uteri (58.3-61.5%) (10.0%). 6 7 decreased (3.2%-19.4%). On other hand, unligated mouse did not decrease (61.1-70.0%). lower 1-5.(ABSTRACT TRUNCATED AT 250 WORDS)
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