Experimental studies on the replication and dissemination of Qalyub virus (Bunyaviridae: Nairovirus) in the putative tick vector, Ornithodoros (Pavlovskyella) erraticus.

摘要: A study was undertaken to determine if the argasid tick, Ornithodoros (Pavlovskyella) erraticus, can serve as a biological vector of Qalyub (QYB) virus. The suckling mice used viremic vertebrate hosts were acceptable for all feeding stages this tick and developed relatively high titered viremias (4.4-6.5 log10PFU/ml) 24-120 hr post intracerebral inoculation. Larval, nymphal, adult ticks became orally infected with QYB virus after ingesting 4.4-6.4 log10PFU/ml. overall infection rate experiments 67/205 recovered up day 179 postfeeding. Incubation known quantities uninfected triturated tissues did not result in any appreciable inactivation. viral antigen detected by immunofluorescence primarily midgut posterior diverticula cells. First second instar nymphs larvae individually transmit mice; however they successfully transmitted when groups 11-20 per mouse. Three out fourteen orally-infected male female mice. Organ titrations exposed demonstrated tissues; dissemination other organ systems discovered only 1 142 days extrinsic incubation. Vertical transmission from progeny demonstrated. Four 39 our colony spirochete; presumably, Borrelia crocidurae. O. (P.) erraticus apparently satisfies conditions that would implicate species is arthropod which has been isolated nature.