An enzyme-linked immunosorbent assay (ELISA) for the measurement of plasmin-alpha 2-antiplasmin complex in human plasma--application to the detection of in vivo activation of the fibrinolytic system.

摘要: An enzyme-linked immunosorbent assay (ELISA) is developed for the measurement of plasmin-alpha 2-antiplasmin complex in human plasma. Microtiter plates were coated with a mixture two murine monoclonal antibodies directed against alpha and bound was quantitated peroxidase-conjugated antibody plasminogen. The lower limit sensitivity 0.01 nM 100-fold diluted plasma, allowing detection 1 undiluted plasma samples. After dilution samples, no longer influenced by presence precursors plasminogen 2-antiplasmin. At concentration 2.0 intra- interassay variation coefficients 4.2 5.5 percent respectively. In samples 25 control subjects levels below nM. Extensive vivo activation fibrinolytic system during thrombolytic therapy streptokinase resulted generation elevated up to 690 +/- 150 No measurable found 32 patients acute deep vein thrombosis nor 11 recurrent thrombosis. These findings indicate that generated its may be useful monitor but not diagnosis venous