The role of steroid hormones in skeletal muscle metabolism

摘要: Steroid hormones play important roles in the regulation of whole body metabolism. Skeletal muscle is an insulin-responsive organ with a key role overall substrate Disturbances skeletal metabolism, as result hormonal imbalance may be underlying defect metabolic disease. Reduced glucose disposal characteristic feature syndrome. The aim this thesis work to identify steroid on and lipid metabolism; dissect impact sex insulin signaling pathways human muscle. A further goal understand how differences Whole metabolism differs between men women, sex-dependent gene expression are evident biopsies. Some retained vitro cultured In contrast, did not show any differences. Chronic exposure cell cultures physiological doses testosterone or 17 β-estradiol resulted responses. Exposure enhanced palmitate oxidation, AMP dependent protein kinase phosphorylation IRS2 myotubes from both sexes, while βestradiol increased oxidation male donors only PDK4 female only. Testosterone treatment insulin-stimulated incorporation into glycogen AKT donors. Acute supra-physiological reduced independent origin cells. Moreover, acute basal disrupted insulin-suppressive effect oxidation. Increased glucocorticoid action leads predispose type 2 diabetes. Local conversion cortisone active cortisol by enzyme 11β-hydroxysteroid dehydrogenase target tissues regulate tissue-specific glucocorticoids patho-physiological states. high dose via induction myotubes. siRNA-mediated reduction pharmacological inhibition HSD1 prevented effects cortisone, but cortisol, conclusion, exert diverse time manner. Modulation hormone actions at specific regulatory steps provide potential therapeutic entry points for disease Type attention should focused understanding disease, sexorigin cells consider when assessing responses culture. ISBN 978-91-7457-377-0