Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer

摘要: Recombinant plasmid constructs are frequently employed in transfection experiments. With the availability of a wide spectrum specialized and versatile eucaryotic cloning/expression vectors, investigators have been given powerful tools to expedite elucidation mechanisms governing gene expression, facilitate identification genes participating diverse cellular processes (namely, metabolism, immune response, differentiation development, repair DNA damage, malignant transformation).