Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer

摘要: The ability to transfect cells with native genomic DNA and engineered gene/ vector constructs has played a leading role in accelerating our understanding of gene regulation function. Yet, despite significant diversification delivery strategies, efforts expand the repertory cell types amenable DNA-mediated transfer continue stumble over recurrent obstacle— namely, sensitivity that recipient often manifest towards procedure itself (1,2) To minimize likelihood cytopalhic effects engendered by chemical physical methods investigators, more more, are adopting low toxicity synthetic polymers ligand-mediated strategies dispatch copious amounts exogenous surfaces target (3—10) Polybrene (Fig. 1) is an amorphous polycation well known retrovirologists for Its augment infectivity retroviral particles nucleic acids vitro (11—13). transfection-enhancing polybrene believed reside m its propensity establish electrostatic bridges between negatively charged viral (or acids) anionic glycoprotein moieties protruding from plasma membranes (11). Inspired elegant simplicity this adsorptive targeting process, Kawai Nishizawa (14) were first report on utility combining dimethyl sulfoxide (DMSO) introduce biologically active (i e., phenotypically selectable) Rous sarcoma into chicken embryo fibroblasts. Shortly thereafter, we (9) others (16-21) demonstrated same basic principle could be applied plasmid, cosmid, or