CHARACTERIZATION OF ADULT HUMAN PROSTATIC EPITHELIAL-CELLS IMMORTALIZED BY POLYBRENE-INDUCED DNA TRANSFECTION WITH A PLASMID CONTAINING AN ORIGIN-DEFECTIVE SV40-GENOME

摘要: Normal adult human prostatic epithelial cells were infected with an adenovirus 12-SV40 virus or transfected by polybrene-induced gene transfer a plasmid (pRSV-T) containing the SV40 early region genes (pRNS-1) origin-defective genome and carrying neomycin resistance gene. Colonies of morphologically altered isolated, cultured in serum-free medium characterized. These had extended lifespan culture compared to normal cells. Both Ad12-SV40-infected pRSV-T-transfected cultures eventually underwent senescence. pRNS-1-transfected (pRNS-1-1), however, have now been grown for more than 50 passages. contain genome, express T-antigen, are not tumorigenic nude mice. They cytokeratins 5 8, like parent cells, pseudodiploid. Analysis growth regulatory processes revealed that pRNS-1-1 was stimulated similarly epidermal factor, insulin-like pituitary extract. The response pRNS-1 growth-inhibitory retinoic acid, also similar However, less responsive inhibition transforming factor-beta, lost altogether ability be inhibited tumor necrosis factor-alpha 1,25 (OH)2 vitamin D3. Therefore transformation appeared alter but - stimulatory mechanisms. should useful elucidating multistep mechanism carcinogenesis prostate.