Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation

作者: Janet H. Parham , Marie A. Iannone , Laurie K. Overton , Jeff T. Hutchins

DOI: 10.1023/A:1008046101653

关键词:

摘要: … CHO-TAg, HEK 293, and 293-EBNA) were measured using electroporation for DNA delivery and … by butyrate treatment post-electroporation yielded increases in fluorescence intensities …

参考文章(27)
R6my A. Aubin, Michael Weinfeld, Marzieh Taghavi, Razmik Mirzayans, Malcolm C. Paterson, Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer Methods of Molecular Biology. ,vol. 62, pp. 319- 342 ,(1997) , 10.1385/0-89603-480-1:319
M Yoshida, M Kijima, M Akita, T Beppu, Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A. Journal of Biological Chemistry. ,vol. 265, pp. 17174- 17179 ,(1990) , 10.1016/S0021-9258(17)44885-X
J.-L. Su, K.E. Kilpatrick, B.R. Champion, D.C. Morris, J.M. Lehmann, T.A. Kost, Fluorescent microtiter screening assay for immunocytochemically reactive antibodies. BioTechniques. ,vol. 22, pp. 320- 324 ,(1997) , 10.2144/97222ST05
J.P. Northrop, K.S. Ullman, G.R. Crabtree, Characterization of the nuclear and cytoplasmic components of the lymphoid-specific nuclear factor of activated T cells (NF-AT) complex. Journal of Biological Chemistry. ,vol. 268, pp. 2917- 2923 ,(1993) , 10.1016/S0021-9258(18)53861-8
T Crook, P J Farrell, G A Parker, M Bain, E A Sara, M J Allday, Epstein-Barr virus nuclear antigen (EBNA)3C is an immortalizing oncoprotein with similar properties to adenovirus E1A and papillomavirus E7. Oncogene. ,vol. 13, pp. 2541- 2549 ,(1996)
A J Strain, W A H Wallace, A H Wyllie, Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells. Biochemical Journal. ,vol. 225, pp. 529- 533 ,(1985) , 10.1042/BJ2250529
Paul F. Lurquin, Gene transfer by electroporation Molecular Biotechnology. ,vol. 7, pp. 5- 35 ,(1997) , 10.1007/BF02821542