The preservation of islet with alginate encapsulation in the process of transportation
作者： Na Li , Ying Zhang , Zhilong Xiu , Yu Wang , Li Chen
DOI: 10.1002/BAB.1295
关键词:
摘要: Restoration of insulin secretion by transplantation isolated islets is a treatment for type Ι diabetes mellitus. One the major issues with clinical islet how to maintain viability during transportation from donor patient. We developed method that uses alginate encapsulation protect mechanical damage shipment. tested several variables their impact on and used significant variable build response surface methodology (RSM) model Box-Behnken design method. This mathematical statistical technique we optimize conditions In this study, factors significantly affected survival rate were incubation time, serum concentration, preservation time. Then, an empirical was built shipping according responses effect RSM. can be predict serve as guide optimizing increasing success transplant procedure.
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