Cryopreservation of freshly isolated porcine islet cells

摘要: Abstract Introduction The use of xenogenic islet cells may be a possibility to overcome the shortage human donor organs treat diabetes. Microencapsulation seems promising method for immunoprotection. Since isolation, purification, encapsulation, and transplantation are labor intensive, cryopreservation has emerged as an attractive system banking. aim this study was determine influence three different freezing media (FM) on viability freshly isolated porcine (FIPIC). Methods FIPIC were using modified Ricordi purification performed Lymphoprep density gradient. Viability prior after thawing determined MTT-based Cell Growth Determination Kit. Insulin production detected enzyme-linked immunosorbent assay. Three FM containing dimethylsulfoxide (DMSO) or glycerol sucrose used cryoprotection FIPIC. Results Isolation resulted in 95% ± 1.3% 97% 1.4% purity. Cryopreservation with I (containing DMEM, FCS, DMSO) yielded 98.4% III glycerol) 93.1% viability, whereas only 85.6% alive when is II DMSO, BM). Glucose stimulation revealed loss 2.8% 1.9% insulin secretion per microgram DNA working III, but decrease glucose-dependent 7.8% ( P Discussion Low concentrations DMSO seem equivalent cryopreserve