Movement of myosin-coated fluorescent beads on actin cables in vitro

作者: Michael P. Sheetz , James A. Spudich

DOI: 10.1038/303031A0

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摘要: Myosin-coated fluorescent beads are observed to move unidirectionally along organized actin filament arrays in the alga, Nitella, with an average velocity similar vivo rates of movement muscle and other cells. The myosin produces motive force, as is ATP dependent blocked by inactivation heads. movement, which occurs absence bipolar thick filaments, provides a quantitative assay for motility.

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