Adenovirus type 12 E1B 19-kilodalton protein is not required for oncogenic transformation in rats.

摘要: Abstract The adenovirus type 12 mutants in700 and pm700 carry site-specific mutations within the reading frame encoding E1B 19-kilodalton protein (19K protein) which prevent production of intact 19K protein. In cultures human A549 cells, these grow just as well wild-type virus does, but they display a large-plaque (lp), cytocidal (cyt) phenotype. DNA in infected cells is not degraded, at late times KB by mutants, degradation (deg) The transformation phenotype also host range. Although are defective for 3Y1 rat cell line, transform mouse primary kidney vitro efficiency capable inducing tumors rats. These results support view that obligatory oncogenic transformation.