Adenovirus type 12 early region 1B proteins and metabolism of early viral mRNAs

摘要: Early region 1B (E1B) of human adenoviruses encodes two major proteins. The 19-kDa polypeptide appears to prevent E1A-induced cytolysis and DNA degradation. larger E1B product approximately 55 kDa, which is essential for viral replication, plays a role in the accumulation stability mRNAs late shutoff host metabolism. For serotype 12 (Ad12), this 482-residue (482R) protein replication. In present report we have used series mutants examine roles Ad12 482R 19-kDa, 163R metabolism early mRNAs. No specific effects on (or late) were detected with any affecting 163R. With mutant dl42, an altered that lacks residues 114-155, both replication synthesis defective. Accumulation E1A transcripts nucleus cytoplasm resembled wt. levels from regions E1B, E2A E3 at later times during infection somewhat lower than those wt, but decrease may been due absence progeny dl42-infected cells. However, E2B E4 all was severely reduced. These data suggested requirement be related its encode products necessary synthesis.