POLYPHENOL OXIDASE INHIBITORY AND ANTIOXIDANT ACTIVITY OF EXTRACT OF CANNA EDULIS KER STEM
作者： JUAN ZHANG , ZHENG WU WANG , QIN MI
DOI: 10.1111/J.1745-4514.2010.00455.X
关键词:
摘要: Polyphenol oxidase (PPO) extracted from Canna edulis Ker rhizome was investigated. The substrate specificity established as catechol > caffeic acid > chlorogenic acid > gallic acid > catechin. optimum temperature and pH of the PPO activity were 20C 6.8, respectively. sensitive to many general inhibitors including C. stem extract. Through response surface design, optimized inhibitor combination, containing 200 mg/L citric acid, 284 mg/L ascorbic 67 mg/L ethylenediaminetetraacetic acid 8,000 mg/L extract, obtained. With 96.73% inhibition rate, combination could be developed a natural additive used in process food preservation sight safety aboveground part confirmed by animal experiment. In addition, increased total phenol content antioxidant rhizome. PRACTICAL APPLICATION In fresh influenced polyphenol oxidase, additives are necessary prevent oxidation which is usually being all fruit vegetable. chemical synthesized always have various side effects it has become hot topic research, develop with activity. However, commonly poor Therefore, good choice study interaction between extract different compounds main component. this study, (including 93.56% extract) obtained through design an effect high 96.73%. preservation.
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