Recellularization of Acellular Human Small Intestine Using Bone Marrow Stem Cells

摘要: We aimed to produce an acellular human tissue scaffold with a view test the possibility of recellularization bone marrow stem cells tissue-engineered small intestine (TESI). Human small-bowel specimens (n = 5) were obtained from cadaveric organ donors and treated sequentially 6% dimethyl sulfoxide in hypotonic buffer, 1% Triton X-100, DNase. Each (SI) piece (6 cm) was recellularized EPCAM+ CD133+ allogeneic cells. Histological molecular analysis demonstrated that after decellularization, all cellular components nuclear material removed. Our also showed decellularized SI retained its histoarchitecture intact villi major structural proteins. Protein films common extracellular matrix constituents (collagen I, laminin, fibronectin) found abundance. Furthermore, several residual angiogenic factors SI. Following recellularization, we viable mucin-positive goblet cells, CK18+ epithelial adjacent muscularis mucosa α-actin+ smooth muscle high repopulation blood vessels CD31+ endothelial results show future, such TESI would be ideal for clinical purposes, because it can derived recipient's own immunocompatible thus avoiding use immunosuppression.