Identification of matrix metalloproteinases 3 (stromelysin-1) and 9 (gelatinase B) in abdominal aortic aneurysm.

摘要: A prominent metalloproteinase activity with an apparent molecular mass of 80 kD and additional activities at 67 through 70, 50, 32 have been observed on casein, gelatin, elastin gel zymography in extracts from abdominal aortic aneurysms (AAAs). The forms 80, were isolated by affinity to recombinant tissue inhibitor metalloproteinases, the 80-kD 50-kD components shown be derived matrix metalloproteinase-9 (MMP-9). relative electrophoretic mobility these under reducing nonreducing conditions corresponds those MMP-9 generated MMP-3 (stromelysin-1) cleavage, active 45 35 detected aneurysmal using specific antibody. Confirmation that major proteolytic is was also demonstrated immunoprecipitation Comparative immunoblots 10 typical AAA patients, antibody against MMP-9, revealed bands 92, 82, 67, 51 53, 27, 23, 20 conditions; six control specimens displayed negligible immunoreactivity. This report first show known activated are present wall they may play a role destruction disease.