Expression of CYP 4A ω-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes.
作者： Debebe Gebremedhin , David X. Zhang , Koryn A. Carver , Nicole Rau , Kevin R. Rarick
DOI: 10.1016/J.PROSTAGLANDINS.2016.04.003
关键词:
摘要: Astrocytes secrete vasodilator and vasoconstrictor factors via end feet processes, altering blood flow to meet neuronal metabolic demand. Compared what is known about the ability of astrocytes release that dilate local cerebral vasculature, very little regarding source identity astrocyte derived constricting factors. The present study investigated if express CYP 4A ω-hydroxylase metabolize arachidonic acid (AA) 20-hydroxyeicotetraenoic (20-HETE) regulates KCa channel activity in arterial myocyte contractility. Here we report cultured 4A2/3 mRNA protein produce 20-HETE epoxygenase metabolites epoxyeicosatrienoic acids (EETs) when incubated with AA. production EETs was enhanced following stimulation metabotropic glutamate receptors (mGluR) on astrocytes. Exogenous application attenuated, whereas inhibition HET-0016 increased open state probabilities (NPo) 71pS 161pS single-channel currents recorded from Exposure isolated myocytes conditioned media caused shortening length freshly not evident synthesis action. These findings suggest only response mGluR but also synthetize constrictor functions as an endogenous inhibitor two types found
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