Quantifiler ® Duo DNA Quantification Kit: A Guiding Tool for Short Tandem Repeat Genotyping of Forensic Samples

摘要: Forensic analysts routinely encounter samples containing mixtures of DNA from male and female contributors PCR inhibitors due to exposure environmental insults. In order select the appropriate STR analysis methodology for such obtain optimal results at first pass, it is desirable determine relative quantities detect presence an early stage in sample processing workflow. Here we describe a multiplex real-time assay that can provide desired information single reaction. Briefly, simultaneous quantification human achieved by measuring RPPH1 target SRY male-specific target. At same time synthetic sequence co-amplified as Internal Control (IPC) inhibitors. The has good dynamic range (0.023–50 ng/μL) 25 pg/μL ten thousand-fold excess DNA. addition, ability predict inhibition was demonstrated shifted IPC C T values increasing hematin. All showed correlation with downstream profiles obtained large set various types therefore demonstrating this be considered guiding tool performance genotyping kits forensic samples.