Recent Advances in Oxygen Measurements Using Phosphorescence Quenching

摘要: Oxygen dependent quenching of phosphorescence is an optical method for measuring oxygen pressure which was first reported in several years ago (see Vanderkooi and Wilson, 1986; Wilson Vanderkooi, 1987). In the intervening has steadily continued to improve both range application precision measurements. The goal present communication review developments have occurred within past two should be significant value investigators interested vitro vivo. There are five major areas improvements and/or extensions occurred: 1. Algorithms been developed permit deconvolution decay curves from probes vascular system tissue into distribution constants (distribution pressures) tissue; 2. New phosphorescent identified suitable absorption emission near infra-red part spectrum (absorptions greater than 620 nm with at less 1,200 nm); 3. Measurements made progressively smaller amounts tissue, measurements 50 μm diameter achieved studies microcirculation Shonat et al, this procedings). 4. Timing circuits allow synchronized rhythmic movements, such as beating heart. This makes it possible, example, obtain digital maps heart vivo Rumsey proceedings). 5. A new needle type phosphorimeter designed a light guide can inserted defined depths tissue. 6. images using confocal techniques (Plant Burns, 1993) technique would expected provide high spatial resolution pressure.