Studies on the electrogenic action of acetylcholine with Torpedo marmorata electric organ

摘要: Abstract When the differential fluorescence emission at 515 nm of receptor-rich membrane fragments from Torpedo marmorata labelled with quinacrine is followed by energy transfer, addition a high concentration an agonist such as 0.4 m -carbamylcholine or -phenyltrimethylammonium causes fast (unresolved) increase intensity slow (minute range) decrease, which leads to final stable level. On other hand, stepwise low concentrations gives only increase. Antagonists, flaxedil and d-tubocurarine, all tested, bring about exclusively increases. Decamethonium no reaction but without transient overshoot. Addition local anaesthetic reduces amplitude response carbamylcholine. The α-toxin Naja nigricollis does not cause any change blocks effect cholinergic agonists antagonists. Dissolution non-ionic detergent abolishes triggered preserves ones observed in presence data are interpreted terms three-state model, revised version model Katz & Thesleff (1957): brought being assigned “activation” receptor-ionophore complex one its “desensitization”.