Chemical genetic analysis of the budding-yeast p21-activated kinase Cla4p

摘要: The p21-activated kinases (PAKs) are effectors for the Rho-family GTPase Cdc42p. Here we define in vivo function of kinase activity budding yeast PAK Cla4p, using cla4 alleles that specifically inhibited by a cell-permeable compound does not inhibit wild-type kinase. CLA4 inhibition cells lacking partially redundant Ste20p causes reversible SWE1-dependent cell-cycle arrest and gives rise to narrow, highly elongated buds which both actin septin tightly polarized bud tips. Inhibition Cla4p prevent polarization F-actin, cytokinesis is blocked only have formed before inhibitor treatment; cell emergence affected inhibition. Although localization necks restored swe1Δ cells, remains defective. delay after polarization, indicating this checkpoint may mediate an adaptive response capable promoting when reduced. Our data indicate required at early stage budding, coincident with formation ring, morphogenesis assembly site.