Emodin suppresses Wnt signaling in human colorectal cancer cells SW480 and SW620.

摘要: Wnt signaling is involved in the regulation of cell proliferation, differentiation and apoptosis. Its aberrant activation a key event pathogenesis progression human colorectal cancers. Dietary phytochemicals are gaining importance as chemotherapeutic agents owing to their potential prevent, delay or reverse oncogenesis. Here we demonstrate that emodin (1,3,8-trihydroxy-6-methylanthraquinone), an anthraquinone present roots bark several medicinal plants, down regulates pathway cancer cells (SW480 SW620) by regulating TCF/LEF transcriptional activity. Emodin significantly regulated expression players (β-catenin TCF7L2) also its various downstream targets (cyclin D1, c-Myc, snail, vimentin, MMP-2 MMP-9). Two novel emodin׳s action were discovered namely co-activator p300 (down regulated) repressor HBP1 (up regulated). Morphological changes induced suggest mesenchymal epithelial transition accompanied increase E-cadherin but marker (alkaline phosphatase) was activated only SW620 (metastatic origin) not SW480 (primary tumor-derived). Moreover, our data indicate reactive oxygen species plays role emodin-mediated inhibition migration induction growth arrest partially rescued scavenger ascorbic acid. Effects shown this study may provide important insights for use complementary integrated medicine treatment cancer.