Telomerase activity as a measure for monitoring radiocurability of tumor cells

摘要: Radiotherapy plays a key role in the treatment of many tumors. It is difficult to determine what fraction tumor cells survives after with ionizing radiation. A convenient and sensitive biochemical assay could be efficacious determining potential success radiotherapy. Since telomerase activity frequently associated malignant phenotype, we sought whether correlation existed between radiation-induced cell killing activity. We evaluated two telomerase-positive one telomerase-negative human line exposed Telomerase was determined using PCR-based telomeric repeat amplification protocol coupled ELISA. found radiation decrease (in plateau phase RKO, HeLa; growing RKO) dose-dependent manner, which correlated death vitro tests as well during regression nude mice. In contr...