Saturable CD14-Dependent Binding of Fluorescein-Labeled Lipopolysaccharide to Human Monocytes

摘要: We used rough lipopolysaccharide (ReLPS) to construct a fluorescein-labeled LPS (FITC-LPS) with very high labeling efficiency that bound isolated human monocytes in CD14-dependent fashion and this respect behaved indistinctively from native LPS. The binding could be inhibited either by 1,000-fold excess of unlabeled or polymyxin B, bactericidal/permeability-increasing protein, cationic protein 18, soluble CD14. Although FITC-LPS preparation no longer possessed the ability prime neutrophils for production reactive oxygen species stimulate produce tumor necrosis factor, activation Limulus amoebocyte lysate cascade was comparable Binding enhanced pooled serum (HPS) LPS-binding (LBP) concentrations up 100 ng/ml completely CD14 dependent. For exceeding ng/ml, still partially dependent, but not HPS LBP association shown totally saturable. In conclusion, we found an HPS- LBP-dependent dependent at ng per ml, saturation shown.