Nitric oxide-loaded chitosan nanoparticles as an innovative antileishmanial platform.
作者： Fernanda V Cabral , Milena T Pelegrino , Ismael P Sauter , Amedea B Seabra , Mauro Cortez
DOI: 10.1016/J.NIOX.2019.09.007
关键词:
摘要: Leishmaniasis is a neglected tropical disease that demands for new therapeutic strategies due to adverse side effects and resistance development promoted by current drugs. Nitric oxide (NO)-donors show potential kill Leishmania spp. but their use limited because of instability. In this work, we synthesize, characterize, encapsulate S-nitroso-mercaptosuccinic acid into chitosan nanoparticles (NONPs) investigate activity on promastigotes intracellular amastigotes (Leishmania) amazonensis. Cytotoxicity macrophages was also evaluated. We verified NONPs reduced both forms the parasite in single treatment. noticed reduction parasitophorous vacuoles as an evidence inhibition growth resolution infection. No substantial cytotoxicity detected macrophages. were able provide sustained killing L. (L.) amazonensis infective stages with no toxicity macrophages, representing promising nanoplatform cutaneous leishmaniasis.
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sci-hub.se 参考文章(51)
Dakota J. Suchyta, Mark H. Schoenfisch, Encapsulation of N-diazeniumdiolates within liposomes for enhanced nitric oxide donor stability and delivery Molecular Pharmaceutics. ,vol. 12, pp. 3569- 3574 ,(2015) , 10.1021/ACS.MOLPHARMACEUT.5B00248
Henry J. C. de Vries, Sophia H. Reedijk, Henk D. F. H. Schallig, Cutaneous Leishmaniasis: Recent Developments in Diagnosis and Management American Journal of Clinical Dermatology. ,vol. 16, pp. 99- 109 ,(2015) , 10.1007/S40257-015-0114-Z
Rogéria C. Zauli-Nascimento, Danilo C. Miguel, Jenicer K. U. Yokoyama-Yasunaka, Ledice I. A. Pereira, Milton A. Pelli de Oliveira, Fátima Ribeiro-Dias, Miriam L. Dorta, Silvia R. B. Uliana, In vitro sensitivity of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Brazilian isolates to meglumine antimoniate and amphotericin B Tropical Medicine & International Health. ,vol. 15, pp. 68- 76 ,(2009) , 10.1111/J.1365-3156.2009.02414.X
Andreas Diefenbach, Heike Schindler, Norbert Donhauser, Elke Lorenz, Tamás Laskay, John MacMicking, Martin Röllinghoff, Ion Gresser, Christian Bogdan, Type 1 Interferon (IFNα/β) and Type 2 Nitric Oxide Synthase Regulate the Innate Immune Response to a Protozoan Parasite Immunity. ,vol. 8, pp. 77- 87 ,(1998) , 10.1016/S1074-7613(00)80460-4
Peter E. Kima, The amastigote forms of Leishmania are experts at exploiting host cell processes to establish infection and persist. International Journal for Parasitology. ,vol. 37, pp. 1087- 1096 ,(2007) , 10.1016/J.IJPARA.2007.04.007
Calvin A. Henard, Eric D. Carlsen, Christie Hay, Peter E. Kima, Lynn Soong, Leishmania amazonensis Amastigotes Highly Express a Tryparedoxin Peroxidase Isoform That Increases Parasite Resistance to Macrophage Antimicrobial Defenses and Fosters Parasite Virulence PLoS Neglected Tropical Diseases. ,vol. 8, pp. e3000- ,(2014) , 10.1371/JOURNAL.PNTD.0003000
Brian C Smith, Michael A Marletta, Mechanisms of S-nitrosothiol formation and selectivity in nitric oxide signaling Current Opinion in Chemical Biology. ,vol. 16, pp. 498- 506 ,(2012) , 10.1016/J.CBPA.2012.10.016
Laura C. Green, David A. Wagner, Joseph Glogowski, Paul L. Skipper, John S. Wishnok, Steven R. Tannenbaum, Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids Analytical Biochemistry. ,vol. 126, pp. 131- 138 ,(1982) , 10.1016/0003-2697(82)90118-X
Xiao-qing Wei, Ian G. Charles, Austin Smith, Jan Ure, Gui-jie Feng, Fang-ping Huang, Damo Xu, Werner Mullers, Salvador Moncada, Fool Y. Liew, Altered immune responses in mice lacking inducible nitric oxide synthase Nature. ,vol. 375, pp. 408- 411 ,(1995) , 10.1038/375408A0
David O. Schairer, Jason S. Chouake, Joshua D. Nosanchuk, Adam J. Friedman, The potential of nitric oxide releasing therapies as antimicrobial agents. Virulence. ,vol. 3, pp. 271- 279 ,(2012) , 10.4161/VIRU.20328