Cloning and characterization of estrogen receptor α in mummichog, Fundulus heteroclitus
作者： Hiroshi Urushitani , Makoto Nakai , Hideko Inanaga , Yasuyuki Shimohigashi , Akio Shimizu
DOI: 10.1016/S0303-7207(03)00118-7
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摘要: Abstract Developmental exposure to 17β-estradiol (E2) induced the death of embryos and fry, malformations, sex reversal, incomplete ossification vertebrae cranial bones in cyprinodont fish, mummichog (Fundulus heteroclitus). To clarify mechanism by which exogenous estrogens caused these developmental effects, we determined sequence an estrogen receptor (ER) coding region, encoded 620 amino acid residues. This region shared 80% identity that ERα medaka (Oryzias latipes). Northern blot analysis showed two mRNAs with 5.5 4 kb were expressed liver. These strongly E2 stimulation. The mRNA was 8 h after treatment, whereas not until 12 Vitellogenin (VTG) stimulation male Receptor binding assays using protein F. heteroclitus (fhERα) ligand domain alkylphenols bind fhERα a higher affinity (50 times or more) as compared human ERα. present results demonstrate has very similar medaka, for this E2-stimulation, followed subsequently VTG expression. Furthermore, more efficiently than
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