Binding units (BU) and the area under binding isotherms (AUI). New indices of effector-target conjugation.

摘要: Abstract New methods for simplified quantitation of effector-target conjugation have been developed. The binding unit (BU) is defined as the number target cells required to bind a specified percentage effector cells. units determined from isotherms in which conjugate frequencies are measured by holding constant and varying Alternately, can be In this case, BU computed at different values Also, area under curve (AUI) these another index that used an overall measure capacity system. experimental AUI agree well with theoretical predictions based on our previously developed model (J. Immunol. Methods (1992) 155, 133–147). relationship between AUI, procedures determine parameters shown. value indices express quantitatively has confirmed determining NK-K562