Central circuits regulating the sympathetic outflow to lumbar muscles in spinally transected mice by retrograde transsynaptic transport

摘要: Despite considerable interest in the mechanisms that control hyperalgesia associated with muscle inflammation, CNS descending pathways coordinate autonomic circuits regulating lumbar muscles are not adequately understood. Here we used both pseudorabies virus (PRV)-614 retrograde transsynaptic tracing and spinally transected method 33 C57BL/6J mice to map polysynaptic between CNS. Tissues were processed for dual-label immunocytochemical detection PRV-614 tryptophan hydroxylase (TPH) or tyrosine (TH)-expressing neurons In intact mice, was transported intermediolateral column (IML) ventral horn (VH) of spinal cord, subsequent transport many brain regions, including medullary raphe nuclei, rostral ventrolateral medulla (RVLM), A5 cell group regions (A5), locus coeruleus (LC), pontine reticular formation nucleus (MRN PRN), paraventricular hypothalamus (PVN), other central sites. However, produced infection IML, main have been shown contribute sympathetic circuits, RVLM, Lateral paragigantocellular (LPGi), A5, LC, PVN, whereas labeling VH MRN eliminated almost every case. above five dual-labeling immunocytochemistry showed coexpression PRV-614/TPH PRV-614/TH immunoreactive (IR) involved these regulatory circuits. Our results reveal a hierarchical organization controlling muscles, thus providing neuroanatomical substrates catecholaminergic serotonergic system regulate muscles.