Environmental Conditions And Agronomic Practices Induce Consistent Global Changes In DNA Methylation Patterns In Grapevine (Vitis vinifera cv Shiraz)

摘要: Fruit attributes that affect wine quality are thought to be largely driven by the interaction of grapevine's genetic characteristics with environmental factors (i.e. climate, soil and topography) vineyard management. All these variables, in conjunction making process, give a its distinctive character. Understanding how grapevines perceive adapt changing environment will provide us an insight into better manage crop quality. Mounting evidence suggests epigenetic mechanisms key interface between genotype ultimately plant's phenotype. Moreover, it is now widely accepted source useful variability during varietal selection could performance. While contribution DNA methylation plant performance has been extensively studied other major crops, very little work done grapevine. Here we used Methylation Sensitive Amplified Polymorphisms obtain global patterns methylation, identify main drivers diversity across 22 vineyards planted cultivar Shiraz six areas zone, The Barossa, South Australia. observed profiles showed high level differentiation grouped their area provenance despite low sub-regions. Furthermore, pairwise distances similar management systems significant correlation geographic distance. Finally, sensitive Genotyping By Sequencing identified 3,598 differentially methylated genes were assigned 1,144 unique GO terms which 8.6% associated response stimulus. Taken together, our results indicate intensity directionality sub-regions within Barossa local growing conditions. discuss can as tool understand potentially modulate terroir