Dynamics and Reversibility of the DNA Methylation Landscape of Grapevine Plants (Vitis vinifera) Stressed by In Vitro Cultivation and Thermotherapy

摘要: There is relatively little information concerning long-term alterations in DNA methylation following exposure of plants to environmental stress. As known about the ratio non-heritable changes and mitotically-inherited changes, dynamics reversibility states were investigated grapevine (Vitis vinifera) stressed by vitro cultivation. It was observed that significant part induced epigenetic could be repeatedly established particular planting stress conditions. However, once conditions discontinued, many gradually reverted returned similar those maternal plants. In fact, period one three years after cultivation it difficult distinguish somaclones Forty percent disappeared within a year subsequent termination ending these probably reflect caused transient reversible stress-responsive acclimation mechanisms. sixty diversity remained 1 represents epimutations. Sequencing regions remaining variable between regenerant revealed 29.3% sequences corresponded non-coding genome. Eight (19.5%) previously identified genes ones (51.2%) annotated as "hypothetical proteins" based on their similarity described other species, including likely undergo (V. vinifera gypsy-type retrotransposon Gret1, auxin-responsive transcription factor 6-like, SAM-dependent carboxyl methyltransferase).