In vivo analysis of DNA methylation patterns recognized by specific proteins: coupling CHIP and bisulfite analysis.

摘要: The three-way connection between DNA methylation, chromatin configuration, and transcriptional regulation is under increasing attention, but the fine rules governing epigenetic control are still poorly understood. In several studies, authors have concluded that methylation status of CpG sites could be critical for binding factors to and, consequently, conformation. We tested possibility a novel technical approach combining immunoprecipitation bisulfite genomic sequencing analysis (ChIP-BA) provide useful information on role specific patterns in driving association vivo proteins given regions. Our results show ChIP-BA permits establishment required methyl-CpG protein addition, can potentially identify do not allow bind Possible fields application discussed. believe wide use make possible exploration aspect intricate web.